STUDIES ON ANALYSIS OF BELLADONNA ALKALOIDS BY CAPILLARY GC AND GC-MSD

A method for analyzing belladonna alkaloids——hyoscyamine,scopolamine, anisodamine and anisodine, by means of capillany GC and GC-MSD wasdescribed. The retention data and characteristic ions of the parent and TMS derivatives ofthese alkaloids are given and will be very useful for identification of unknown alkaloids.The derivatization of different silylation reagents was compared, and the derivatizationwith MSTFA was found to be better than with BSA and BSTFA. The method has beenused for studying biotransformation of these alkaloids in biosynthesis. Analysis ofanisodamine and anisodine by CGC and GC-MS has not been found in the literature Inaddition, the advantages of TMS derivatization are also discussed.