PHOSPHORIMETRIC ANALYSIS OF CARBAINDOLINE

Carbaindoline possesses strong phosphorescence with excitation at 304 mm and emission at 434 nm in water at 77K. The phosphorescence is stronger in alkaline media than in acid media. KI enhances the phosphorescence of carbaindoline but shortens the life time. The heavy cations Cu²⁺ , Fe³⁺, Pb²⁺, Tl³⁺, Zn²⁺, and molybdate do not enhance the phosphorescence, but some of them, Cu²⁺ and Fe³⁺, have quenching effect on carbaindoline phosphorescence. The phosphorescence of nine carbaindoline analogues was studied. After oxidation of>CH₂ at position 2 to>C= O, the phosphorescence decreased significantly.The phosphorimetric method for analysis of carbaindoline was established with a detection limit of 5 ng/ml. Ten minutes after ip administration of carbaindoline 0.1 mg/kg to mice, the blood drug concentration was found to be 149±27ng/ml.