Protective effect of (-)clausenamide against neurotoxicity induced by okadaic acid and β-amyloid peptide25-35
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Abstract
This study is to investigate the protective effect of (-)clausenamide against the neurotoxicity of okadaic acid in SH-SY5Y cell line, and injection β-amyloid peptide25-35 (Aβ25-35) to the cerebral ventricle in ovariectomy (OVX) rats. MTT assay, LDH assay, and Hoechst 33258 staining were used to detect the effect of (-)clausenamide on the toxicity of okadaic acid in SH-SY5Y cell line. The animal model was induced by ovariectomized and injection of Aβ25-35 in the cerebroventricle of rats. The effect of (-)clausenamide on learning and memory deficiency was observed by step-through test. Electron microscope, Nissl body staining, and HE staining were used to examine the morphological changes in hippocampus and cerebral cortex neurons. Pretreatment of (-)clausenamide and LiCl decreased the rate of cell death from MTT, LDH release, and apoptosis from Hoechst 33258 staining in SH-SY5Y cell line. The step-through tests showed (-)clausenamide could improve the ability of learning and memory. The Nissl body staining and HE staining experiments also showed the neuroprotective effects of (-)clausenamide on the neurons of hippocampus and cerebral cortex. (-)Clausenamide has the protective effects against the neurotoxicity induced by okadaic acid and Aβ25-35.
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