XQ Zhang, AR Liu , LX Xu, . DETERMINATION OF RANUNCULIN IN PULSATILLA CHINENSIS AND SYNTHETIC RANUNCULIN BY REVERSED PHASE HPLCJ. Acta Pharmaceutica Sinica, 1990, 25(12): 932-935.
Citation: XQ Zhang, AR Liu , LX Xu, . DETERMINATION OF RANUNCULIN IN PULSATILLA CHINENSIS AND SYNTHETIC RANUNCULIN BY REVERSED PHASE HPLCJ. Acta Pharmaceutica Sinica, 1990, 25(12): 932-935.
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DETERMINATION OF RANUNCULIN IN PULSATILLA CHINENSIS AND SYNTHETIC RANUNCULIN BY REVERSED PHASE HPLC

    Abstract
  • A method for the determination of ranunculin in Pulsatilla chinensis and synthetic ranunculin by reversed phase HPLC using betamethasone as internal standard is described. The column employed was a 10μm LiChrosorb RP - 18 (230×4mm), the eluting solvent consisted of methanol-water(40: 10, V/V)and the effluent was monitored at 225nm.The advantages of thismethod are simple, rapid and accurate. The coefficients of variation are less than 1%.
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