INHIBITORY EFFECTS OF INDOMETHACIN AND MELOXICAM ON NF-κB IN MOUSE PERITONEAL MACROPHAGES

AIM　To study the inhibitory effects of indomethacin and meloxicam on NF-κB from lipopolysaccharide (LPS) stimulated peritoneal macrophages of mice. METHODS　NF-κB was measured with the method of electrophoretic mobility shift assay (EMSA). RESULTS　After induction by LPS at the concentrations of 1 and 3 μg.mL^-1, the NF-κB content of the mouse peritoneal macrophages increased markedly. Indomethacin and meloxicam, at the concentrations of 10^-7-10^-5 mol.L^-1, decreased the activation of NF-κB at the concentrations of 1 and 3 μg.mL^-1 in activated mouse peritoneal macrophages induced with LPS at the concentrations of 1 and 3 μg.mL^-1. CONCLUSION　The inhibitory effects of indomethacin and meloxicam on NF-κB activation may be one of their mechanisms of antiinflammatory actions.