Separation and identification of active constituents of Paris vietnamensis

AimTo study the anti-tumor bioactive steroid saponins of Paris vietnamensis (Takht.). MethodsThe constituents were isolated and purified by chromatography and their structures were identified by spectral analysis and physicochemical properties. The cytotoxic bioactivities of the constituents were determined by MTT. ResultsEleven compounds were obtained and identified as 3β,5α,6α-trihydroxy-7(8)-en-isospirostanol-3-O-β-D-glucopyranosyl(1→3)［α-L-rhamnopyranosyl(1→2)］-β-D-glucopyranoside (1), which was named as parisvietnaside A, 25(R)diosgenin-3-O-α-L-arabinofuranosyl(1→4)-β-D-glucopyranoside (2), 25(R)diosgenin-3-O-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside (3), 25(R)diosgenin-3-O-α-L-arabinofuranosyl(1→4)［α-L-rhamnopyranosyl(1→2)］-β-D-glucopyranoside (4), 25(R)diosgenin-3-O-β-D-glucopyranosyl(1→3)［α-L-rhamnopyranosyl(1→2)］-β-D-glucopyranoside (5), 25(R)diosgenin-3-O-α-L-rhamnopyranosyl(1→4)-α-L-rhamnopyranosyl(1→4)［α-L-rhamnopyranosyl-(1→2)］-β-D-glucopyranoside (6), 25(R)pennogenin-3-O-α-L-arabinofuranosyl(1→4)-β-D-glucopyranoside (7), 25(R)pennogenin-3-O-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside (8), 25(R)pennogenin-3-O-α-L-arabinofuranosyl(1→4)［α-L-rhamnopyranosyl-(1→2)］-β-D-glucopyranoside (9), 25(R)pennogenin-3-O-β-D-glycopyranosyl(1→3)［α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside (10) and 25(R)pennogenin-3-O-α-L-rhamnopyranosyl(1→4)-α-L-rhamnopyranosyl(1→4)［α-L-rhamnopyranosyl-(1→2)］-β-D-glucopyranoside (11). Some constituents had cytotoxic bioactivities. ConclusionCompound 1 is a new spirostanol saponin, and compounds 2, 3, 6-11 were obtained from Paris vietnamensis (Takht.) for the first time. Compounds 3, 4, 6, 8 had cytotoxic bioactivities against tumor cells HepG2 and SGC-7901.