Pharmacokinetics and distribution of superoxide dismutase liposomes in rats

AimTo evaluate the effects of surfactants on the pharmacokinetics and distribution in rats after intravenous administration of SOD liposomes. MethodsThe liposomes were prepared by reverse phase evaporation method. The activity of SOD was assayed by method of xanthine oxidase. ResultsThe T_1/2 of SOD solution, common SOD liposome, SOD liposomes modified by DSPE-PEG2000 and Tween 80 were 0.25, 0.34, 0.66 and 0.41 h, respectively; AUC were 12.48, 24.66, 41.16 and 33.02 μg·h·mL^-1, respectively. Compared with the common liposome, the liposomes modified by DSPE-PEG and Tween 80 decreased the content of SOD in liver and spleen, but increased in brain. ConclusionThe three kinds of liposomes could increase T_1/2 and AUC in some extent， especially in PEG-L group. Tween-L could increase the SOD content in brain, and PEG-L could decrease the SOD content in the liver and spleen compared with the common liposome.