DETERMINATION OF FIVE ISOFLAVONES IN BELAMCANDA CHINENSIS BY RP-HPLC

A reversed phase high performance liquid chromatographic method for thequantitative determination of the five isoflavones(tectoridin,iridin,tectorigenin,irigenin andirisflorentin)in the rhizome of Belamcanda and Iris was developed.The column was packed withTSK gel ODS 80 TS(150 mm×4.6 mm).Methanol-water(36%～60%)was used as mobile phase.The method was rapid,sensitive, precise and has good reproducibility.The five isoflavones in the crude drngs were completely separated within 40 min,Recoveries ofthe five constituents were 98.41 %～101.93 %, The crude drugs were analysed as follows:Thepowdered sample was refluxed with methanol for 6 hours. The extract was transferred into a 2 mlvolumetric flask and diluted to mark with methanol.A definite amount of this sample solution wasinjected into RP-HPLC.The contents of the five constituents in the rhizomes of Belamcanda and Iris were calculated from the relevant peak heights or peak areas.The analytical results of determination for various species and habitats are shown in Tab 2～5.