INHIBITORY EFFECTS OF M-NIFEDIPINE ON ANGIOTENSIN ⅡSTIMULATED PROLIFERATION AND PROTEIN SYNTHESIS IN CULTURED RABBIT AORTIC SMOOTH MUSCLE CELLS

By using rabbit aortic smooth muscle cells(VSMC)in culture as a model,theeffects of m-nifedipine(m-Nif)on the growth of VSMC were observed.The results showed that m-Nif inhibited ³H-thymidine and³H-leucine incorporation into DNA and protein,ecreased cellnumber in a concentration-dependent manner in angiotensinⅡ(ANGⅡ)induced VSMC.At theconcentration of 2 μmol·L^-1,m-Nif was found to reduce the ANGⅡ(100 nmol·L^-1)stimulated³H-thymidine,³H-leucine incorporation and cell number by 46%,58% and 53%,respectively。Our results suggest that m-Nif can inhibit ANG Ⅱ stimulated proliferation and protein synthesis incultured rabbit aortic VSMC.