DETECTION OF SUPEROXIDE AND HYDROXY RADICALS FORMED IN BLEOMYCIN A₆-Fe²⁺ SYSTEM BY SPIN TRAPPING

The active radicals produced in Bleomycin A₆-Fe²⁺ system have been studied by combination of spin trapping technique with ESR spectroscopy. It was found that the superoxide radicals could be detected by NOS in the above system. The hydroxyl radicals could be trapped by PBN in aqueous solution. In accordance with the parameters of ESR spectra of spin adducts of PBN-OH in aqueous and methyl alcohol solutions, the production of OH radical have been further demonstrated.