INVESTIGATION OF THE SELECTIVE METHOD FOR OPTIMAL COMBINATION OF PAPER CHROMATOGRAPHIC SOLVENT SYSTEMS OF 22 KINDS OF AMINO ACIDS

The optimal combination of paper chromatographic solvent systems of 22 kinds of amino acids was made successfully with the stepwise combination method on TRS-80 microcomputer. Three groups of optimal combination of solvent systems were selected. The numbers of optimal combination system are 7, 4, 2, 1; 17, 9, 4, 2; 21, 17, 4, 2. The 22 kinds of amino acids tested could be separated at a level that the difference of Rf × 100 values between any two samples is equal to 10 or more using each combination of the groups mentioned above. At the same time, numerical taxonomic technique-information content method was also used to select the optimal combination of the systems, but no satisfactory results were obtained.