EFFECT OF GONADOTROPIN-RELEASING PEPTIDE (GRP) ON LH SECRETION OF MOUSE PITUITARY IN VITRO

Synthetic GRP and its analogues ,GRP-NH₂,Glu^7.9.14 Lys^6.10 GRP(6～14),Phe¹⁴GRP(5～14)andPhe¹⁴GRP,at the concentration of 0.05 mmol·L^-1 were shown tohave stimulatory effect on LH secretion in cultured mouse pituitary in vitro. The luteotropin releasingactivity of GRP and its analogues was estimated to be 115.4,114.2,140.0,162.0 and 179.0%ofthe control group,respectively. Subcutaneous administration of Phe14GRP on days 7～9 and 1～5 of pregnancy and phe¹⁴GRP(5～14)on days 2～4 of gestation at dosage of 1 mg·d^-1(eachmouse)caused fetal death in 40～60% of mice.