STUDIES ON THE STYLE OF INTERACTION OF PIRARUBICIN WITH DNA BY FLUORESCENCE SPECTRA

AIM: To study the characteristics of the interaction of pirarubicin (THP) with DNA. METHODS: Study and determination of DNA with THP as a fluorescence probe was presented by absorption and fluorescence spectra. RESULTS: The binding constant of the interaction between THP and DNA is 1.66×105 L.mol^-1, and the binding site number is about 2.6 base pairs. The limit of detection is 0.91 μg.mL^-1 and the linear ranges are 0.91～9.0 μg.mL^-1 and 9.0～26 μg.mL^-1 respectively for the determination of DNA. CONCLUSION: It indicates that the anthracycline moiety of THP intercalates the stacked base pairs of DNA, which is the major controlling factor. In addition, the complex sugar structure of THP may be bound to the minor groove of DNA double helix.