Isolation and structure identification of lignans from pine needles of pinus massoniana Lamb.

AimTo study the chemical constituents from the water-extracts of pine needles of Pinus massoniana Lamb. MethodsChromatographic techniques were used to separate and purify compounds. Their physico-chemical properties and spectral data (UV, IR, MS, ¹H-¹H, 13C-¹H NMR, DEPT, HMBC etc.) were used to elucidate the structures. ResultsThree lignans were isolated from the N-BuOH fraction of water-extracts. Their structures were identified as (7S,8R)-3′,4,9′-tridihydroxy-4-methoxy-9-O-shikimoyl-7,8-dihydrobenzofuran-1′-propylneolignan (massonianoid A, I), (7S,8R)-4,9-dihydroxy-3,3′-dimethoxy-7,8-dihydrobenzofuran-1′-propylneolignan (II) and 4,4′,8-trihydroxy-4,4′-dimethoxy-9-lignanolide (III). ConclusionCompound I is a new compound. While II and III were isolated from this plant for the first time.