THE EXPRESSION LEVELS OF GIRK1 AND IRK1 IN MEMORY DEFICIT RATS

AIM To study the expression level of GIRK1 and IRK1 in cerebral cortex after aggregated beta amyloid peptide_25-35 injection into the rat cerebral ventricle for different times. METHODS To test the spatial learning and memory in Morris water maze. To observe GIRK1 and IRK1 expression levels by RT PCR. RESULTS In Morris water maze task, the latencies of beta amyloid peptide treated rats were longer than those of the sham rars in the 4, 5, 8, 10 training periods (“student”t-test, P<0.05). The expression levels of GIRK1 in beta amyloid peptide injection day 1 and day 3 groups are decreased significantly compared with sham group (Tukey's test, P<0.05) and there is no difference between beta amyloid peptide injection day 17 group and sham group. IRK1 expression levels are no difference among the four groups. CONCLUSION Injection of aggregated beta amyloid peptide_25-35 into rat cerebral ventricles can induce memory and potassium channel expression changes. Beta amyloid peptide induced inhibition of GIRK1 expression may have some relations with brain dysfunction.