STUDIES ON NEW CYTOTOXIC ANNONACEOUS ACETOGENINS FROM UVARIA GRANDIFLORA AND ABSOLUTE CONFIGURATIONS

Two new cytotoxic annonaceous acetogenins, named uvarigrin(1) and uvarigrandin A(3), were obtained from the roots of Uvaria grandiflora Roxb(Annonaceae). Based on X-ray analysis and Mosher's methodology, the overall absolute configuration of 1 was established as 15S,17R,18R,21R,22R,36S.The absolute configuration of 3 was also resolved by Mosher'smethodology. The relative configuration of the previously reported uvarigranin(2) was revised. Compound 1 showed cytotoxicity against HCT-8, Bel₇₄₀₂ and A₂₇₈₀ human tumor cell lines at ED₅₀ levels of 0.15, 0.21 and 0.41μg·ml^-1, respectively.