THE FLUOROMETRIC METHOD FOR ANALYSIS OF SOME ACID DRUGS

By using salicylate as a working drug, pyronine was chosen from 31 dyes to form drug-dye complex. In Na₂HPO₄ solution, salicylate reacts with pyronine to form a complex which is soluble in chloroform. Pyronine fluoresces strongly at λ_ex 542 nm and λ_em 566 nm and the intensity of pyronine in complex is proportional to the amount of salicylate in the complex with detection limit of 5 ng/ml.By using this method, suberogorgin, flufenamic acid, mechlofenamic acid, mefenamic acid,sodium tauroglycocholate and trichloroacetic acid may be determined with the sensitivity of 10～50 ng/ml.Salicylate in blood was determined by this method with the sensitivity of 10 ng/ml blood. Good recovery and precision were obtained.