Studies on change of chemical composition in Coptis-Scute herb couple by using HPCE and HPLC

Quantitative analysis on different proportions of couple of Coptis and Scute. The proportions of Coptis and Scute couple were 6∶1, 6∶4, 6∶7, 6∶10, 6∶13, separately. The contents of alkaloids in Coptis and decoction of Coptis and Scute were determined by HPCE and the contents of flavones in Scute and decoction of Coptis and Scute were determined by HPLC, separately. Precipitates were generated after the couple of Coptis and Scute, which results the decrease of contents of effective ingredients.