Investigation on the hydroxylation metabolism of imrecoxib in vitro by using recombinant buman CYPs

To identify the drug-metabolizing enzymes involved in the hydroxylation of the new anti-inflammatory and anodyne in recoxib. Methods imtabolites and remained parent drug were detected with liquid chromatography-multistage mass spectrometry. The contribution of 4 CYPs in the hydroxylation metabolism of imrecoxib was evaluated by total nomalized rate (TNR) method. Results Imrecoxib is metabolized by CYP2C9, CYP2D6 and CYP3A4,with the rate of 62.5%, 21.2% and 16.4%, respectively. Conclusion CYP2C9 is the major enzyme involved in imrecoxib hydroxylation metabolism.