RP-HPLC DETERMINATION OF LOGANIN IN MOUSE PLASMA AND ITS PHARMACOKINETICS

AIMTo develop a method for determination of loganin in mouse plasma by using high-performance liquid chromatography. The method was employed to study pharmacokinetics of loganin. METHODSAn RP-C18 was used as the stationary phase.The mobile phase consisted of methanol-water (30∶70), at the flow-rate of 0.8 mL·min^-1. The UV absorbance detector was set at 240 nm. Plasma samples were treated with solid phase extraction. RESULTS The recovery of loganin in mouse plasma was 86.0%～91.5%. The calibration curve in plasma was linear over the range of 0.01～5.00 μg·mL^-1. The limit of quantitation was 10 ng·mL^-1. The RSDs of intra-day and inter-day (N=5) were less than 15%. The pharmacokinetic parameters were C_max=6.8 μg·mL^-1, _tmax=30 min, _t1/2α=26.1 min, _t1/2β=29.01 min. CONCLUSIONThe method is accurate, sensitive and suitable for pharmcokinetic study of loganin. The absorption and elimination of loganin were rapid after ig in mice.