INHIBITORY EFFECTS OF SHIKIMIC ACID ON PLATELET AGGRAGATION AND BLOOD COAGULATION

AIM：To study the inhibitory effects of shikimic acid (SA) on platelet aggregation and its mechanism, and the effect on coagulation time. METHODS: Methods of turbidimetry and radioimmunoassay were used to measure platelet aggregation and the levels of thromboxane B₂(TXB₂) and 6-keto-PGF1α. RESULTS: SA inhibited rabbit platelet aggregation induced by ADP and collagen in vitro with IC₅₀ of 9.25 and 3.56 mmol.L^-1, respectively. SA 25 and 50 mg.kg^-1 ip for 3 d inhibited platelet aggregation induced by ADP in rats subjected to middle cerebral artery thrombosis(MCAT). SA was shown to increase rat plasma 6-keto-PGF_1α and no significant effect on TXB2 generation in PRP induced by ADP. SA 25, 50 and 100 mg.kg^-1 im or iv elevated the blood coagulation time of mice.　CONCLUSION: SA inhibited platelet aggregation and blood coagulation.