Determination of chlorogenic acid and eriodictyol-7-O-β-D-glucuronide in Pyrrosia by RP-HPLC

Aim To develop a method for determination of chlorogenic acid and eriodictyol-7-O-β-D-glucuronide in Pyrrosia of different species and different places of origin by RP-HPLC. Methods Chromatography was performed using a C₁₈ column with mobile phase of methanol-water-phosphoric acid (50∶200∶0.2). The monitoring wavelength was 284 nm. Results The linear ranges were 0.01-5.0 μg (R=0.999 7) and 0.004-5.0 μg (R=0.999 7), the recoveries were 97.1% (N=8, RSD=2.7%) and 98.8% (N=9, RSD=2.5%) for chlorogenic acid and eriodictyol-7-O-β-D-glucuronide, respectively. Conclusion The method was employed to the analysis of 21 samples of Pyrrosia. The contents of compounds vary greatly depending on the species used, place of collection and time of harvesting. The HPLC method is sensitive, rapid and can be used to control the quality of Pyrrosia and to guide reasonable season of harvesting.