Preparation of cardiomyocyte-targeting liposomes and their targeting ability in virto

AimTo prepare the cardiomyocyte-targeting liposomes and investigate their cardiomyocyte targetability in virto. MethodsLiposomes modified with compound PAC were prepared (PAC-L); The uptake of PAC-L by cardiomyocytes was studied by incubating fluorescence labeled liposomes with cardiomyocytes in virto and measuring the association of liposomes by a fluorescence spectrophotometer. ResultsA high affinity of PAC-L to the cardiomyocytes was observed, the amount of cell uptake of PAC-L by cardiomyocytes was higher than that by nonmyocyte (P<0.001); The amount of cardiomyocyte uptake of PAC-L on the normoxia condition 2 h was 2.9-fold higher than that of plain-L, and the increase was 5.2-fold when hypoxia occured; The form of liposome uptake changed, the amount of cardiomyocyte uptake of Plain-L by internalization was only 11%, while that of PAC-L was 56%. ConclusionIt is indicated that PAC-L was a potential drug carrier for targeting to ischemic myocardium.