STUDIES ON POLYPEPTIDES Ⅸ.SYNTHESIS AND ANTIGEN SPECIFICITY OF PRE-S REGION FRAGMENTS OF HEPATITIS B SURFACE ANTIGEN

Seven peptide fragments of HBsAg Pre-S region have been synthesizedby liquid phase and solid phase methods. All peptide fragments were linked to protein car-rier by carbodiimide and glutaraldehyde methods. The antigen specificity of thepeptide-protein conjugates was assayed by anti-HBsAg polyclonal antibodies and anti-"a" monoconal antibody. P₁ and P₅ were found to have higher antigen specificity.