Comparison of the pharmacokinetics of lidamycin in mice determined by two methods

AimTo compare two methods, the total radioactivity assay (RA method) and the radioactivity assay after separation with high performance liquid chromatography (HPLC-RA method). Methods¹²⁵I-Lidamycin was prepared by Iodogen method and separated by size exclusive high performance liquid chromatography. The pharmacokinetic parameters of lidamycin were assayed by two methods after intravenous injection to mice at the dose of 100 μg·kg^-1, and compared by statistical analysis. ResultsThe pharmacokinetic parameters (_Vd, t_1/2α, t_1/2β, k₂₁, k₁₀, k₁₂, AUC and CL) showed significant difference between the two methods (_P<0.05). ConclusionThe HPLC-RA method was better than the RA method to determine unchanged ¹²⁵I-lidamycin.