ISOLATION, PURIFICATION AND PHYSICO-CHEMICAL PROPERTIES OF IMMUNOACTIVE CONSTITUENTS FROM THE FRUIT OF LYCIUM BARBARUM L.

Three glycoconjugates, LbGp3, LbGp4 and LbGp5, were isolated from the fruit of Lycium barbarum L. Molecular weights of LbGp3, LbGp4 and LbGp5 were 9.25×10⁴, 21.48×10⁴ and 2.37×10⁴, respectively. Carbohydrate contents of LbGp3, LbGP4 and LbGp5 were 93.6%, 85.6%, 8.6%, respectivly. LbGp3 was composed of Ara and Gal in a molar ratio of 1∶1. LbGp4 was composed of Ara, Gal, Rha and Glc in a molar ratio of 1.5∶2.5∶0.43∶0.23. LbGp5 was composed of Rha, Ara, Xyl, Gal, Man and Glc in a molar ratio of 0.33∶0.52∶0.42∶0.94∶0.85∶1. Elemental analysis of N contents: LbGp3 0.83%, LbGp4 1.72%, LbGp5 9.58%. The linkage between the glycan and protein may be of O-linkage in LbGp4.