DETERMINATION OF INDIGO AND INDIRUBIN IN QINGDAI BY HPLC

A HPLC method has been established to analyze indigo, and indirubin in Oingdai under the following conditions. Column: LiChrosorb RP-18 10μm, 2.6×250mm; flow rate: 1.5ml/min; detector: LC-65T (Perkin-Elmer) at 292 nm; sensitivity: 0.02 aufs.Tryptanthrin was used as the internal standard and peak height ratio or peak area ratio was used for quantitative determination This method showed good linearity (r=0.999. n=5) in the range of 2.4～12 μg/ml. The average recovery of indigo is 99.07～103.70% with CV<2% (n=3), and that of indirubin is 99.49～101.23% with CV<2% (n=3).This method is sensitive, rapid, simple and showed good reproducibility.