REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETER MiNATION OF TIAPRIDE IN PLASMA AND URINE

Plasma or urine samples, containing metoclopramide as an IS were extracted at pH 10.58 With dichloromethane The concentrated extract was analysed by HPLC (YSB-Ⅰ Model, China) on a column (150 × 5 mm)oF YWG-C₁₈H₃₇ (10 μm, Tianjin) with methanol-triethylamine solution (10000:0.40) as mobile phase (1.0 ml/min) and detected at 254 nm The retention time of tiapride and internal standard were 4.5 and 8.25 min respectively. The correlation coefficients of the extracted tiapride calibration curves were over 0.99. The minimal detectable concentration of tiapride in serum was 0.05 μg/ml. The recoveries of tiapride in serum and urine were over 98%. The CV of Within-day and day-to-day were less than 2.5%. The results of this method were correlated with that of the ILC method. The HPLC method for detection of tiapride in plasma or urine is highly sensitive, accurate, reproducible and rapid, so it could be applicable for extensive clinical and pharmacological investigations.