RPHPLC METHOD FOR DETERMINATION OF ISORHYNCHOPHYLLINE IN PLASMA AND ITS PHARMACOKINETICS IN RABBITS

A reversed phase HPLC method for determination of isorhnchophylline (IRHY) in rabbit plasma was developed. A Shimadzu LC-10A liquid chromatograph equipped with a Shimadzu SPD-10A multiwavelength detector and a Chromatopac LC-10A data processor were used. The analytical column was a 4.6 mm×150 mm CLC-ODS. The mobile phase was a mixture of methanolwater (95∶5), the flow rate was 1 ml·min^-1. Detection was performed at UV 254 nm. The calibration curve was linear in the range from 0.016 to 16 μg·ml^-1 with γ=0.9990. The lowest detection limit was 0.016 μg·ml^-1. The precision and accuracy of withinday and day-to-day ranged from 3.8% to 6.9%. The recoveries were 80.5% to 85.1%. The drug concentrtion-time curves of IRHY in rabbits after iv of 2 mg·kg^-1 and 5 mg·kg^-1 were shown to fit a two-compartment open model with half-times of 1.32±0.6 h and 1.25±0.09 h respectively. The absorption was fast after intraduodenal administration of IRHY (2 mg·kg^-1 and 5 mg·kg^-1) to rabbits with half-times of 1.75±0.18 h and 1.26±0.20 h, respectively. The bioavailability of IRHY was about 42.4% to 69.4%. The results showed that the method was simple and fast. The absorption and elimination were fast atfer intraduodenal administration or iv of IRHY to rabbits.