Diterpene constituents of Tripterygium willfordii (Ⅱ)

In order to study the constituents and pharmacology of Tripterygium plants (Tripterygium willfordii Hook.f), a variety of chromatography methods were used.  Four compounds were isolated from Tripterygium plant and their structures were elucidated by UV, IR, MS, HR-MS, ¹H NMR, ¹³C NMR and 2D-NMR techniques.  The isolated compounds were named as triptonide (1), neo-triptetraolide (2), 2α-hydroxytriptonide (3), and 15-hydroxytriptonide (4), separately.  Compounds 3, 4 belong to new diterpenoids, which can inhibit the growth of K562 cells (leukemia cells) and HL60 cells (acute myeloid leukemia cells).