Isolation and structure identification of chemical constituents from the seeds of Descurainia sophia (L.) Webb ex Prantl

AimTo isolate and determine the structures of chemical constituents from the seeds of Descurainia sophia (L.) Webb ex Prantl. MethodsThe chemical constituents were extracted from the seeds of Descurainia sophia (L.) Webb ex Prantl with 75% ethanol and purified by polyamide, silica gel, RP-C₁₈ and Sephadex LH-20 on column chromatography. Chemical methods and spectroscopic methods, such as ¹H and 13CNMR, HSQC, HMBC and TOCSY spectra were used for the structural identification. ResultsFifteen compounds were obtained. Twelve of them were identified as quercetin-3-O-β-D-glucopyranosyl-7-O-β-gentiobioside (I), kaempferol-3-O-β-D-glucopyranosyl-7-O-β-gentiobioside (II), isorhamnetin-3-O-β-D-glucopyranosyl-7-O-β-gentiobioside (III), quercetin-7-O-β-gentiobioside (IV), kaempferol-7-O-β-gentiobioside (V), isorhamnetin-7-O-β-gentiobioside (VI), quercetin-3,7-di-O-β-D-glucopyranoside (VII), kaempferol-3,7-di-O-β-D-glucopyranoside (VIIIIX), kaempferol-3-O-β-D-glucopyranosyl-7-O-［(2-O-trans-sinnapoyl)-β-D-glucopyranosyl(1→6)］-β-D-glucopyra-noside) (X), sinapic acid ethyl ester (XI) and 3,4,5-trimethoxyl-cinnamic acid (XII). Conclusion Compounds X and VI are new compounds.IV, V, VII, VIII and IX were isolated from Cruciferae family for the first time. I, II, III were obtained from Descurania genus and XI, XII from D.sophia for the first time.