EFFECTS OF ISORHAPOTIGENIN AND RESVERATROL ON FUNCTION OF PERIPHERAL BLOOD POLYMORPHONUCLEAR LEUKOCYTES FROM RABBITS

I n effects of isorhapotigenin and resveratrol on chemotaxis and release of β-glucuronidase from rabbit peripheral blood polymorphonuclear leukocytes stimulated with formylmethionyl-leucyl-phenyl-phenylalanine(fMLPP) were studied. The optimal concentration of fMLPP for the induction of chemotaxis of polymorphonuclear leukocytes was approximately at 5×10^-10 mol·L^-1. fMLPP, at the concentration range of 10^-10～10^-6 mol·L^-1, dose-dependently increased the release of β-glucuronidase from polymorphonuclear leukocytes. Isorhapotigenin and resveratrol both inhibited the chemotaxis of polymorphonuclear leukocytes stimulated with fMLPP(5×10^-10 mol·L^-1). Isorhapotigenin, at the concentration range of 10^-5～10^-4 mol·L^-1, dosedependently inhibited the release of β-glucuronidase from polymorphonuclear leukocytes stimulated with fMLPP(10^-8 mol·L^-1). Resveratrol, at the concentrations of 1×10^-5 and 3×10^-5 mol·L^-1, was shown to dosedependently inhibit β-glucuronidase release from polymorphonuclear leukocytes stimulated with fMLPP(10^-8mol·L^-1).