Part II: Design, synthesis and antitumor action of C3/C3 bis- fluoroquinolones linked-cross 2, 5-1, 3, 4oxadiazole

To develop a new small molecular probe for discovering an antitumor lead compound from the replacement of carboxylic group of two molecular antibacterial fluoroquinolones with a heterocyclic ring, a   series of the C3/C3 bis-fluoroquinolones tethered with an 1, 3, 4-oxadiazole ring were synthesized as their    respective HCl salts, and their structures were characterized by elemental analysis and spectral data.  The     in vitro antitumor activity against L1210, CHO and HL60 cell lines was also evaluated via the respective IC₅₀ values by methylthiazole trazolium (MTT) assay.