SOLIDPHASE EXTRACTION COUPLED WITH HIGH-FIELD PROTON NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY: APPLICATION TO THE ANALYSIS OF BRODIMOPRIM METABOLITES IN RAT URINE

The use of coupled solid-phase extraction and high-field proton nuclear magnetic resonance spectroscopy (SPE¹HNMR) for the detection and identification of the urinary metabolites of brodimoprim is described. Urine was obtained from Wistar rats following a dose of 200 mg·kg^-1 of brodimoprim. Analysis was performed on partially purified extracts obtained by solid-phase extraction onto C-18 bonded silica gel. As each spectrum was often given by a mixture of more than two kinds of metabolites, stepwise identification of the individual metabolites was achieved by sorting out signal sets having invariant proportionality. Five kinds of metabolites of the drug were found by the analysis.