DETERMINATION OF STERIUDAL SAPONINS IN RHIZOMA PARIDIS BY RP-HPLC

A reversed phase HPLC method for the separalion and determination of nine steroidal saponins in Rhizoma paridis is deseribed The column WaS Symnletry C₈，mobile phase Was aeelonilrile—water(42：58)，UV detection WaS performed at 203 nm The calibration curves showed good linlearity over lhe range of 0.8～12.0 ug，γ=0.9996～0.9999．the recoveries were 97.1％～100.2％for the nine steroidal saponins The method is simple，fast，sensitive and accuraie and has been applied to the analysis of Rhizoma paridis. The results showed that the eontenis of the nine steroidal saponins in Rhizoma paridis were different with different sourees.