Effects of baicalin against oxidative stress injury of SH-SY₅Y cells by up-regulating SIRT1

This study is to investigate the effect of baicalin (BL) against oxidative injury stress of SH-SY₅Y cells induced by H₂O₂ and the possible mechanism.  SH-SY₅Y cells were pre-incubated with baicalin (25, 50, and 100 μmol·L⁻¹) for 12 h prior to exposure to H₂O₂ (150 μmol·L⁻¹) for 24 h.  The viability of SH-SY₅Y cells was measured by MTT assay.  The contents of LDH and NO were determined.  The percentage of apoptotic cells was assessed by flow cytometry (FCM).  The content of Caspase-3 was tested by immunofluorescence histochemical method.  BL at 50 and 100 μmol·L⁻¹ separately increased the cell viability and up-regulated SIRT1, reduced the contents of LDH, NO, Caspase-3 and the apoptotic percentage of SH-SY₅Y cells.  This study results suggest that baicalin could inhibit the H₂O₂-induced neuronal apoptosis.  The further mechanism studies show that baicalin inhibit apoptosis via reducing Caspase-3 expression and up-regulating SIRT1.