EFFECTS OF ISOPRENALINE ON APOPTOSIS RELATED GENE EXPRESSION IN RAT MYOCARDIUM CELLS

AIM　To study the relationship between acute myocardial ischemia rat induced by isoprenaline (Iso) and expression of some apoptosis related genes in myocardium. METHODS　Acute myocardial ischemic rat model was induced by subcutaneous injection of large doses of Iso. The myocardial expression of apoptosis related genes, Fas, CPP32, Bcl-2 and Bax, were detected with RT-PCR method. The influences of aloperine (Alo, 20 mg.kg^-1) and propranolol (Prop, 2 mg.kg^-1) on apoptosis related genes were also investigated by the same method. RESULTS　Myocardial expression of Fas, CPP32 and Bcl-2 were increased in acute myocardial ischemic rats, but no significance was detected in Bax expression. Both Alo and Prop were shown to decrease the expression of Fas and CPP32, and increase expression of Bcl-2. Prop also showed down regulated effect on expression of Bax. CONCLUSION　Subcutaneous injection of large doses of Iso was shown to induce expressions of apoptosis related genes in rat myocardium, which were also influences by Alo and Prop.