Study of a novel compound 2460A with activities produced by fungus

With IL-6R as target, a new compound 2460A was identified from fungus using HTS screening model.  The taxonomics of the produced strain was confirmed to be Trichoderma hazianum rifai after sequencing analysis of rDNA-ITS (internal transcribed spacer).  Results showed that this compound has a binding activity on IL-6R competed with IL-6, thus it is a new ligand of IL-6R originating from microbe.  With MTT assay, the anti-tumor activities of 2460A were demonstrated on CM126 and HT-29 cell lines separately, the IC₅₀ are 2.17×10⁻⁵ mol?L⁻¹ and 1.8×10⁻⁵ mol?L⁻¹ respectively.  The compound affected lightly the HT-29 cell cycle at S phase.  Studies for the anti-tumor activity of 2460A in vivo are in progress in our lab.