HPLC AND TLC-DENSITOMETRY DETERMINATION OF ALKALOIDAL CONSTITUENTS IN SOPHORA FLAVESCENS AND SOPHORA LOPECUROIDES

This paper deals with two analytical methods (HPLC and TLCdensitometry) of alkaloids in Sophora flavescens Ait. and Sophora alopecuroides Linn. Sophocarpine (Ⅰ), matrine (Ⅱ), sophoridine (Ⅲ), oxymatrine (Ⅳ) and oxysophocarpine (Ⅴ) all were fairly separated either on alkaline silica gel G thin layer by step-wise development or on HPLC silica gel column with two mobile phases. Cinchonidine and atropine were chosen as internal standard respectively and quantified by peak height ratio in the HPLC method.Both methods are sensitive, simple and rapid. The CV% are 1.62～2.77% and 0.6～1.74% respectively. Analytical results obtained by these two methods are in good agreement.