EFFECT OF SODIUM FERULATE ON ARACHIDONIC ACID METABOLISM

Sodium ferulate (SF)is one of the antiplatelet ingredients in Radix Aneglica sinensis. The effect of SF on ¹⁴C-arachidonic acid metabolism in washed intact rabbit platelets was studied with radiochromatography and radioautography. SF(0.1～3.2 mmol/L) inhibited the generation of platelet thromboxane B₂ in a dose-dependent manner (reduced by 16.7～93.8'%)and the IC₅₀ was shown to be 0.762 mmol/L. Simultaneously with the reduction of TXB₂ generation, the formation of PGE₂ and PGF_2α was also/educed significantly after treatment with SF. Using mdioimmunoassay SF (0.145～2.32 mmol/L) was found to inhibit rabbit platelet TXB₂ formation in a dose-dependent manner. SF (0.58～2.32 mmol/L)also suppressed aortic tisssue 6-keto-PGF_1α generation in rabbits. At the same concentrations the inhibitory effect of SF on platelet TXB₂ formation was greater than that on aortic tissue 6-keto-PGG_1α genetation. These results indicate that the cyclo-oxygenase activity may be inhibited by SF.