WANG Jie, YANG Song-cheng, WU Sheng-ming, WANG Hong-xia, WEI Kai-hua, ZHANG Xue-min. Application of PSD-MALDI-TOF-MS to protein identificationJ. Acta Pharmaceutica Sinica, 2004, 39(8): 627-630.
Citation: WANG Jie, YANG Song-cheng, WU Sheng-ming, WANG Hong-xia, WEI Kai-hua, ZHANG Xue-min. Application of PSD-MALDI-TOF-MS to protein identificationJ. Acta Pharmaceutica Sinica, 2004, 39(8): 627-630.
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Application of PSD-MALDI-TOF-MS to protein identification

    Abstract
  • AimTo identify protein spots on two dimentional protein electrophoresis (2DE) by post-source decay (PSD) technique associated with library search. MethodsThe PSD-MALDI-TOF-MS method was set up by a segment of ACTH and a peptide digested by trypsin for TPA. ResultsTwo unknown protein spots on 2DE were identified as 40S ribosomal protein S12 and dnaK suppressor protein separately by established PSD-MALDI-TOF-MS method. ConclusionPSD technique has greater application prospects in peoteomics.
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