Changes of gene expression profiles in CCl₄ injured liver of mice

AimTo study the gene expression profiles between the CCl₄ injured liver and normal liver in mice, and to screen the differentially expressed genes that relate to liver injury by CCl₄ on a large scale using cDNA microarrays. MethodsMale Kunming strain mice were divided into two groups: one was control group and another was CCl₄ injured liver group that was given 0.1% CCl₄ oil solution ip at dose of 10 mL·kg^-1 every three days, totally for ten times. Then mRNA in livers of the two groups of mice was extracted, separately, and reversely transcribed to cDNA with the incorporation of different fluorescent-labeled dUTP as the hybridization probes. The mixed probes were hybridized to the cDNA microarrays. The fluorescent signal values were acquired by scanner and analyzed with statistical software. ResultsAmong the 14 100 target genes, 379 genes were differentially expressed, in which 163 genes were up-regulated and the other 216 genes were down-regulated. They are closely related to a range of biological functions. ConclusionUsing the cDNA microarray and experimental animal modeling technique, the differentially expressed genes of CCl₄ injured liver in mice on a large scale could be studied. It is useful for further investigation of the injury mechanism of CCl₄.