DETECTION OF IMPURITIES IN DNA SAMPLES BY USING THE CONVOLUTION SPECTRUM METHOD

AIM　To detect the presence of phenol, amino acids and endotoxin in DNA preparations. METHODS　By using the convolution curve transformation technology, correlation coefficient discriminant which was put forward by spectrum space theory and computer information process technology, impurities in DNA samples were detected. RESULTS　The convolution spectrum method compared with A₂₆₀/A₂₈₀ ratio method and second-derivative absorption spectrum method could be used for the semiquantitative determinations of impurities in DNA samples and the results were satisfactory. CONCLUSION　The method was simple, convenient, fast and valuable to the biochemical analysis.