REVERSED PHASE HPLC DETERMINATION OF THREE COMPONENTS IN SALVIA MILTIORRHIZA INJECTIONS

This paper describes the separation of the three components, danshensu (Ⅰ), protocatechuic acid (Ⅱ) and protocatechuic aldehyde (Ⅲ) in Salvia miltiorrhiza injection and compound Salvia miltiorrhiza injection by reversed-phase HPLC and ion suppression technique, and the determination of these components by external standards on the basis of peak height. Stationary phase was octadecylsilane silica 10 μm, mobile phase was composed of water-methanol-glacial acetic acid (80:19:1). Flow rate was 1.5 ml/min. UV detector was used. The samples were analyzed with Waters μBondapak C₁₈ column (3.9×300 mm), Radial Pak Cartrige C₁₈ column (8×100 mm) and YWG C₁₈ column (5×150 mm) (Chinese Academy of Sciences, Shanghai, Scientific Appratus Manufactory) respectively, and satisfactory results were obtained. Sensitivities were compared, when four channel UV detector and fixed wavelength 254 nm UV detector were used. Recoveies of the compounds added to the sample were 99.6%, 100.4% and 99.2% for Ⅰ, Ⅱ and Ⅲ respectively, and the coefficients of variation were 1.1%, 1.9% and 0.9%. The results for ten samples were given.