Proteomic study of the effect of recombinant hFGF-10 adenovirus on HaCat cells

This study is to investigate the effect of recombinant hFGF-10 adenovirus on the proteome     of HaCat cells, and to speculate further the possible mechanism of the effect of hFGF-10 on HaCat cells via  differentially expressed proteins identified.  Two-dimensional gel electrophoresis (2-DE) combined with tandem time-of-flight mass spectrometry was applied to identify the differentially expressed protein spots on the 2-DE maps of the whole-cell proteins from Ad infected and rAd-hFGF-10 infected HaCat cells.  The mRNA and  protein levels of the differentially expressed proteins were confirmed with semi-quantitative RT-PCR and   Western blotting.  The results showed that the 2-DE maps with high resolution were obtained, and four selected differentially expressed proteins involved in cell apoptosis, cytoskeleton regulation and protein degradation  were identified with MALDI-TOF/TOF.  The mRNA and protein levels of one of the differentially expressed proteins, VDAC2, were up-regulated in HaCat cells infected with the recombinant hFGF-10 adenovirus.  The differentially expressed protein, VDAC2, may be related to the bioactivities of hFGF-10.