EFFECTS OF DYNORPHIN A (1-8) ON CELL CYCLE AND TOTAL CELLULAR PROTEIN DURING NEURONAL AGING IN VITRO

Serum-free culture of mouse neuroblastoma cells was usetd as the experimentalmodel for the study of neuronal aging , with flow cytometry of cell cycle , DNA and total cellularprotein as the indices of neuronal aging.After addition of dynorphin A(1-8)10^-7mol·L^-1 into theculture medium, the following general tendencies were obtained:(1) The number of S and G₂+Mphase cells was increased and the number of G_{1 phase cells decreased.(2) The total cellular protein inaged experimental neural cells decreased. The results implies that Dyn A( 1-8) Produced effects oncell cycle, DNA and total cellular protein in the delaying neuronal aging. The relationbetween Dyn A(1-8) and neuronal aging mierits further investigation.}