Determination of daurisoline in rabbit plasma and study on its pharmacokinetics

AimTo establish an HPLC method for the determination of daurisoline (DS) in rabbit plasma and investigate its pharmacokinetic characteristics after intravenous administration. MethodsDauricine was used as internal standard. The plasma samples were deproteinated with acetonitrile and extracted with two-step dichloromethane. Acetonitrile-water-triethylamine (18∶82∶0.28, pH 3) was used as mobile phase at a flow rate of 1.0 mL·min^-1. The UV-detector was set at 284 nm. ResultsThe linear range was 0.05-20.00 μg·h·mL^-1 with correlation coefficients 0.999 6. The limit of quantitation (LOQ) was 0.05 mg·L^-1 of plasma. The absolute and relative recoveries were above 80% and (101±5)%, respectively. The intra- and inter-assay coefficient of variation were 1.9%-5.6% and 3.5%-6.5%, respectively. The plasma concentration-time profiles were adequately described by a two-compartment open model. ConclusionA sensitive, precise and accurate method for the determination of DS in plasma was described, which can be used in its pharmacokinetic studies.