Mechanisms of sodium ferulate inhibition of collagen synthesis in hepatic stellate cells

AimTo study the mechanisms of sodium ferulate (SF) on inhibition of collagen synthesis in hepatic stellate cells. MethodsCollagen synthesis was analyzed by measuring ³H-proline incorporation. ELISA method was used to study the effect of SF on transforming growth factor β₁ (TGFβ₁) level in cultured HSC-T6 cell. The effect of SF on the TGFβ₁ activity in the supernatant of culture was analyzed by mink lung epithelial cell (Mv1Lu) proliferation inhibition by MTT assay. ResultsSF inhibited collagen synthesis in hepatic stellate cells stimulated with TGFβ₁. SF was shown to decrease TGFβ₁ level in the supernatant of HSC-T6 increased by oxidative stress. TGFβ₁ activity was intervened by SF. ConclusionSF could decrease collagen synthesis, with mechanism may be associated with that SF intervened TGFβ₁ activity, and reduced the level of TGFβ₁ increased by oxidative stress.