EFFECTS OF PRO-INFLAMMATORY CYTOKINES AND LEUKOTRIENS ON PRODUCTION OF TNFα FROM MURINE PERITONEAL MACROPHAGES

AIM: To investigate production of TNFα from murine peritoneal macrophages stimulated with inflammatory mediator, such as IL-1, IL-6, IL-8, LTB₄, LTC₄ and LTD₄. METHODS: L929 cytotoxicity was used to show the level of released and cell-associated TNFα in murine peritoneal macrophages measured by means of crystal violet staining assay. RESULTS: rhIL-8(40～4 000 U.mL^-1) was shown to induce dose-dependent increase of released TNFα from murine macrophages. rmIL-1β(0.001～1μg.mL^-1) was found to increase the level of released TNFα dose-independently, whereas rhIL-6(10～10 000 U.mL^-1), LTB4(5.64×10^-9～5.64×10^-7mol.L^-1), LTC4(1.73×10^-9～1.73×10-7mol.L^-1), LTD4(4.03×10^-9～4.03×10^-7mol.L^-1) did not induce macrophages to produce TNFα. They all can not elevate cell-associated TNFα. CONCLUSION: The results suggest that rhIL-8 and rmIL-1 can induce production of TNFα from murine peritoneal macrophages, while rhIL-6, LTB₄, LTC₄ and LTD₄ can not induce production of TNFα　from murine peritoneal macrophages.