Triterpenes from fruits of Paliurus ramosissimus

To study the chemical constituents of the fruits of Paliurus ramosissimus, various chromatographic techniques were used to separate and purify the chemical constituents.  Three triterpenes have been isolated and purified by using various column chromatography.  Their structures were elucidated by their physico-chemical properties and spectroscopic data.  These compounds were determined as: 22S, 23R-epoxy-tirucalla-7-ene-3α, 24, 25-triol (1), 21S, 23R-epoxy-21, 24S, 25-trihydroxy-apotirucalla-7-ene-3-one (2), 21R, 23R-epoxy-21-ethoxy-24S, 25-dihydroxy-apotirucalla-7-ene-3-one (3), separately.  Compound 1 is a new compound, and the others were obtained from this genus for the first time.